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| SALLES, H. O.; POMPEU, R. C. F. F.; VIEIRA, L. da S.; CAVALCANTE, A. C. R.; SOUZA, H. A. de; GUEDES, F. L.; OLIVEIRA, E. L. de; DIAS, M. V.; MENDES, M. E. P.; XIMENES, L. V.. |
| Produzir novos insumos e de estratégias de baixo impacto ambiental para controle da verminose se mostram como as principais demandas do setor para reduzir a perda de animais e a presença de resíduos químicos nos produtos de origem animal e no ambiente. |
| Tipo: Comunicado Técnico (INFOTECA-E) |
Palavras-chave: Econemat; Nematóide; Helminto Gastrintestinal; Verminose; Controle Integrado; Parasito de Animal; Parasitose; Parasitismo; Manejo do Solo; Haemonchus Contortus; Gastrointestinal nematodes; Nematode control; Nematoda; Soil management; Animal parasitic nematodes; Parasitism; Parasitoses; Brazil. |
| Ano: 2018 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1099750 |
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| Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva. |
| Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018 |
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| Santos,Thaís Rabelo dos; Lopes,Welber Daniel Zanetti; Buzulini,Carolina; Borges,Fernando de Almeida; Sakamoto,Cláudio Alessandro Massamitsu; Lima,Roberto Cesar de Araújo; Oliveira,Gilson Pereira de; Costa,Alvimar Jose da. |
| Seventy-six naturally infected bovines, males and females of mixed breed, aged 8 to 12 months-old, were necropsied. The results of necropsy revealed the presence of 9 helminth genera and 16 species, with the following prevalence and mean infection intensity: Haemonchus placei (100.0%; 3895.5); Haemonchus similis (29.0%; 159.6); Cooperia punctata (100.0%; 5595.0); Cooperia spatulata (32.9%; 137.8); Cooperia pectinata (34.2%; 1010.5); Trichostrongylus axei (69.7%; 239.2); Trichostrongylus colubriformis (10.5%; 10.8); Trichostrongylus longyspicularis (2.6%; 0.5); Ostertagia ostertagi (2.6%; 3.1); Ostertagia lyrata (2.6%; 1.5); Ostertagia trifurcata (1.3%; 0.3); Oesophagostomum radiatum (94.7%; 470.9); Trichuris discolor (47.4%; 32.5); Strongyloides papillosus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prevalence; Gastrointestinal nematodes; Bovine. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400029 |
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| Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann. |
| T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012 |
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